260/280


The ratio of absorbance at 260 nm and 280 nm is used to assess the purity of DNA and RNA.

A ratio of ~1.8 is generally accepted as “pure” for DNA; a ratio of ~2.0 is generally accepted as “pure” for RNA.

If the ratio is appreciably lower in either case, it may indicate the presence of protein, phenol or other contaminants that absorb strongly at or near 280 nm.

Thursday, January 28, 2010

DNA, mRNA, RNA, proteins, RNA molecules, protein, nucleotides, cells, genes, tRNA, ribosome, cell, uracil, enzymes, protein synthesis, amino acids, enzyme, molecule, transcription, synthesis
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